1 00:00:05,430 --> 00:00:03,510 the dragon cargo ship that is launching 2 00:00:07,510 --> 00:00:05,440 today is carrying a variety of supplies 3 00:00:09,589 --> 00:00:07,520 for the station and its crew members 4 00:00:11,430 --> 00:00:09,599 including several new experiments some 5 00:00:14,789 --> 00:00:11,440 of which are designed to learn more 6 00:00:17,150 --> 00:00:14,799 about how plants grow in weightlessness 7 00:00:18,790 --> 00:00:17,160 one of them is known by the acronym cara 8 00:00:21,429 --> 00:00:18,800 c-a-r-a 9 00:00:23,830 --> 00:00:21,439 and uh the principal investigator dr 10 00:00:25,029 --> 00:00:23,840 analisa paul of the university of 11 00:00:26,870 --> 00:00:25,039 florida is on the phone with me this 12 00:00:28,550 --> 00:00:26,880 morning to talk about that 13 00:00:30,630 --> 00:00:28,560 so it's an exciting day today because 14 00:00:32,069 --> 00:00:30,640 we've got this uh cargo craft that's 15 00:00:34,470 --> 00:00:32,079 going up and we've got we know we've got 16 00:00:35,910 --> 00:00:34,480 several uh lots of supplies and several 17 00:00:38,150 --> 00:00:35,920 experiments one of those experiments i 18 00:00:39,910 --> 00:00:38,160 wanted to talk to you about is cara we 19 00:00:42,069 --> 00:00:39,920 said this is an acronym can you tell us 20 00:00:43,670 --> 00:00:42,079 what cara stands for and generally what 21 00:00:45,110 --> 00:00:43,680 you are trying to find out in this 22 00:00:47,670 --> 00:00:45,120 investigation 23 00:00:51,029 --> 00:00:47,680 sure so cairo stands for characterizing 24 00:00:51,990 --> 00:00:51,039 arabidopsis root attractions and it is 25 00:00:54,150 --> 00:00:52,000 um 26 00:00:56,389 --> 00:00:54,160 flying a type of plant called 27 00:00:58,630 --> 00:00:56,399 arabidopsis phthaliana which is 28 00:01:00,549 --> 00:00:58,640 essentially a you know it's a model 29 00:01:02,709 --> 00:01:00,559 organism for plants it's what you might 30 00:01:05,429 --> 00:01:02,719 think of as the white mouse of the plant 31 00:01:07,910 --> 00:01:05,439 world and it is utilized in a lot of 32 00:01:09,270 --> 00:01:07,920 different ground studies as well as 33 00:01:11,030 --> 00:01:09,280 we've run a number of different space 34 00:01:13,030 --> 00:01:11,040 flight experiments with arabidopsis in 35 00:01:15,749 --> 00:01:13,040 the past what we're trying to find out 36 00:01:19,190 --> 00:01:15,759 with this particular experiment is some 37 00:01:21,270 --> 00:01:19,200 of the nuances that plants use to sense 38 00:01:23,350 --> 00:01:21,280 their environment as they grow 39 00:01:25,030 --> 00:01:23,360 without the cues that you normally have 40 00:01:27,190 --> 00:01:25,040 in place and so on the ground of course 41 00:01:29,510 --> 00:01:27,200 you've got gravity which 42 00:01:31,590 --> 00:01:29,520 roots growth towards gravity and shoots 43 00:01:34,230 --> 00:01:31,600 the leaf part grows away from gravity 44 00:01:36,710 --> 00:01:34,240 and the other major cue for growing on 45 00:01:37,830 --> 00:01:36,720 the ground is of course light and so 46 00:01:39,990 --> 00:01:37,840 what we're trying to do with this 47 00:01:42,310 --> 00:01:40,000 particular experiment is we are taking 48 00:01:44,710 --> 00:01:42,320 gravity out of the equation and looking 49 00:01:46,630 --> 00:01:44,720 how plants grow on the surface of these 50 00:01:47,749 --> 00:01:46,640 square auger plates 51 00:01:49,830 --> 00:01:47,759 um 52 00:01:51,910 --> 00:01:49,840 when the light source isn't a directed 53 00:01:54,630 --> 00:01:51,920 one as opposed to 54 00:01:57,270 --> 00:01:54,640 coming just from one direction and 55 00:01:59,910 --> 00:01:57,280 seeing how the roots and shoots grow in 56 00:02:01,830 --> 00:01:59,920 that very diffuse lighting environment 57 00:02:04,230 --> 00:02:01,840 of just slapped up against the bulkhead 58 00:02:06,149 --> 00:02:04,240 of the space station with uh with no 59 00:02:07,429 --> 00:02:06,159 gravity also to give them give them 60 00:02:10,150 --> 00:02:07,439 direction 61 00:02:11,990 --> 00:02:10,160 so tell me also we were just looking at 62 00:02:13,830 --> 00:02:12,000 a couple of pictures of these plants 63 00:02:15,670 --> 00:02:13,840 what kind of plants are you flying and 64 00:02:17,430 --> 00:02:15,680 then why did you pick them for this 65 00:02:19,510 --> 00:02:17,440 particular experiment 66 00:02:21,270 --> 00:02:19,520 so so they said these are arabidopsis 67 00:02:22,790 --> 00:02:21,280 phthaliana that's the genus and the 68 00:02:25,910 --> 00:02:22,800 species of these plants we usually just 69 00:02:27,830 --> 00:02:25,920 call them arabidopsis for short the um 70 00:02:30,309 --> 00:02:27,840 we chose those because as they said they 71 00:02:32,949 --> 00:02:30,319 are the model organism for plants they 72 00:02:35,110 --> 00:02:32,959 we know um everything about their genome 73 00:02:38,470 --> 00:02:35,120 it's been completely sequenced it's very 74 00:02:40,309 --> 00:02:38,480 well characterized on the the uh 75 00:02:42,229 --> 00:02:40,319 in ground experiments it's been used for 76 00:02:44,229 --> 00:02:42,239 just decades and decades in plant 77 00:02:45,589 --> 00:02:44,239 physiology plant molecular biology 78 00:02:48,070 --> 00:02:45,599 research 79 00:02:50,390 --> 00:02:48,080 in addition rob ferl and i my colleague 80 00:02:51,990 --> 00:02:50,400 in the in this business have done a 81 00:02:54,710 --> 00:02:52,000 number of different space flight 82 00:02:57,190 --> 00:02:54,720 experiments with this same plant and so 83 00:02:59,589 --> 00:02:57,200 every time we do an experiment we get 84 00:03:02,070 --> 00:02:59,599 answers and those answers then develop 85 00:03:03,830 --> 00:03:02,080 into new hypotheses and new questions 86 00:03:05,589 --> 00:03:03,840 and so the experiment that we're we're 87 00:03:07,910 --> 00:03:05,599 launching today with these plants is 88 00:03:09,830 --> 00:03:07,920 actually based on information that we 89 00:03:11,990 --> 00:03:09,840 got from the previous space flight 90 00:03:14,390 --> 00:03:12,000 experiment with these same type of type 91 00:03:16,070 --> 00:03:14,400 of plants tell me how will the station 92 00:03:17,750 --> 00:03:16,080 crew members be actually participating 93 00:03:19,750 --> 00:03:17,760 in the in the experiment what will they 94 00:03:23,110 --> 00:03:19,760 be doing with the plants 95 00:03:24,550 --> 00:03:23,120 well in two ways um one is that we 96 00:03:27,430 --> 00:03:24,560 part of what we would like to do is 97 00:03:29,589 --> 00:03:27,440 we're looking at the 98 00:03:31,990 --> 00:03:29,599 molecular biology of these plants and so 99 00:03:33,990 --> 00:03:32,000 not just how the roots grow but what 100 00:03:36,229 --> 00:03:34,000 governs the way they grow 101 00:03:37,830 --> 00:03:36,239 and part of the mechanism to do this is 102 00:03:39,509 --> 00:03:37,840 we've engineered these plants with 103 00:03:40,550 --> 00:03:39,519 something called the green fluorescent 104 00:03:43,350 --> 00:03:40,560 protein 105 00:03:46,070 --> 00:03:43,360 reporter gene that is 106 00:03:48,309 --> 00:03:46,080 essentially reports back to where 107 00:03:51,509 --> 00:03:48,319 certain genes are being engaged as the 108 00:03:53,270 --> 00:03:51,519 plant grows and develops it glows green 109 00:03:55,750 --> 00:03:53,280 but you have to have a special mechanism 110 00:03:57,990 --> 00:03:55,760 to see the fluorescence you can't just 111 00:04:00,309 --> 00:03:58,000 see it in regular light and so one of 112 00:04:02,630 --> 00:04:00,319 the things that we can do is use a 113 00:04:04,869 --> 00:04:02,640 device called the lmm for light 114 00:04:06,309 --> 00:04:04,879 microscopy module that's on station it's 115 00:04:08,070 --> 00:04:06,319 essentially a 116 00:04:10,470 --> 00:04:08,080 very sophisticated fluorescent 117 00:04:13,030 --> 00:04:10,480 microscope that is run 118 00:04:14,710 --> 00:04:13,040 by a glenn research center and so what 119 00:04:16,629 --> 00:04:14,720 the astronaut will do is they'll take 120 00:04:18,069 --> 00:04:16,639 one of the plates and there's there's 121 00:04:19,590 --> 00:04:18,079 there's a number of plates that are 122 00:04:21,590 --> 00:04:19,600 being flown but they'll take one of the 123 00:04:23,990 --> 00:04:21,600 specific plates that has been has some 124 00:04:26,629 --> 00:04:24,000 of these engineered plants on it and put 125 00:04:29,189 --> 00:04:26,639 it on the light microscopy module 126 00:04:30,870 --> 00:04:29,199 apparatus and then what we will do is 127 00:04:32,070 --> 00:04:30,880 we'll be traveling to glen research 128 00:04:35,110 --> 00:04:32,080 center to 129 00:04:37,909 --> 00:04:35,120 then use the microscope 130 00:04:40,550 --> 00:04:37,919 up there to manipulate what's on station 131 00:04:43,110 --> 00:04:40,560 and so the astronaut will put the plate 132 00:04:45,510 --> 00:04:43,120 on the microscope but then it will be 133 00:04:47,350 --> 00:04:45,520 managed by some of the engineers and 134 00:04:50,790 --> 00:04:47,360 scientists at glenn research center as 135 00:04:52,629 --> 00:04:50,800 we see what is glowing on orbit 136 00:04:54,469 --> 00:04:52,639 thank you so much for talking with me